Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adrenal glands
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - mouse kidney tissue
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - human adipose tissue
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Breast
Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Placenta
Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bronchi
The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.
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