siRNA / miRNA gene silencing Human H1299

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1

Get tips on using siRNA P300 to perform siRNA / miRNA gene silencing Human - BOSC23 p300

Get tips on using siRNA CBP to perform siRNA / miRNA gene silencing Human - BOSC23 CBP

Get tips on using siRNA ADAM17 to perform siRNA / miRNA gene silencing Human - BOSC23 ADAM17

Get tips on using siRNA AURKA to perform siRNA / miRNA gene silencing Human - A549 AURKA

Get tips on using siRNA EGFR to perform siRNA / miRNA gene silencing Human - A431 EGFR

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HCT-116 VDAC1

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - Min-6 VDAC1