Get tips on using FITC BrdU Flow Kit to perform Cell cycle assay mouse - RAW 264.7
Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media RAOEC
Get tips on using LC3B Mouse Monoclonal Antibody (9H5) to perform Autophagy assay cell type - Ramos
Get tips on using Anti-LAMP1 antibody [1D4B] (ab25245) to perform Autophagy assay cell type - RAW 264.7
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Ramos
Get tips on using APO-BRDU™ Kit (RUO) to perform TUNEL assay cell type - Rabbit synovial fibroblasts
Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - RAW 264.7
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - Raw 264.7
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