Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using siGENOME Human ARAP1 (116985) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ARAP1

Get tips on using siGENOME Human TBX2 (6909) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U251 TBX2

Get tips on using siGENOME Human BRCA1 (672) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - ES2 BRCA1

Get tips on using siGENOME Human DAB2 (1601) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 DAB2

Get tips on using siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M244 PD-L2

Get tips on using siGENOME Human PPARD (5467) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PPAR-delta

Get tips on using siGENOME Human ATG12 (9140) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Atg12

Get tips on using siGENOME Human RAB5A (5868) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Rab5a

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.