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Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Yeast - Candida albicans

The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method.  For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay Caco-2

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Ca Ski

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Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using MasterPure™ Yeast RNA Purification Kit to perform RNA isolation / purification Yeast - Candida albicans