siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using ON-TARGETplus Human CYP3A4 (1576) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP3A4

Get tips on using ON-TARGETplus Human CYP2B6 (1555) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP2B6

Get tips on using siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M244 PD-L2

Get tips on using siGENOME Human PPARD (5467) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PPAR-delta

Get tips on using ON-TARGETplus Human ITGB4 (3691) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 ITGB5

Get tips on using ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 DPAGT1

Get tips on using ON-TARGETplus Human CTHRC1 (115908) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - CAL-27 CTHRC1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using ON-TARGETplus Human ARL2 (402) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa ARL2

Get tips on using ON-TARGETplus Human ARL3 (403) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ARL3