When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Liver
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Kidney
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Uterus
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Esophagus
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Adipose
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Human Joints
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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