Protein expression and purification Yeast Saccharomyces cerevisiae

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Hello fellow Labettors, I would like to know what would be the best method/medium to reactivate my dormant Lactobacillus paracasei cells?. Any suggestions are greatly appreciated.

Discussions Reactivate dormant Lactobacillus Paracasei

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Discussions Floxing mice with CRISPR

Get tips on using Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728 to perform ChIP Anti-bodies H3K27me2

Products Cell Signaling Technology Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728

Get tips on using Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326 to perform ChIP Anti-bodies H3K4me1

Products Cell Signaling Technology Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Discussions How can I preserve leukocytes for DNA methylation profiling?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - BMDM

Products Thermo Fisher Scientific 7-AAD (7-Aminoactinomycin D)

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - PC-3

Products Thermo Fisher Scientific 7-AAD (7-Aminoactinomycin D)

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion ATDC5 Nfatc1

Products Addgene lentiCRISPR v2
pX330A-1x3 Product

Get tips on using pX330A-1x3 to perform CRISPR Mouse - Deletion ATDC5 Perk

Products Addgene pX330A-1x3

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