Cell line authentication

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCtAEC

Get tips on using Endothelial Cell Growth Medium MV to perform Mammalian cell culture media BAOEC

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - SKOV3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - RAW 264.7

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - T47D