siRNA / miRNA gene silencing Human HNSCC cell line

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Mouse - Liver

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Liver

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat liver tissue

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue

Get tips on using SYTO™ 9 Green Fluorescent Nucleic Acid Stain to perform Live / Dead assay bacteria - Pseudomonas aeruginosa

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Primary cells Rat schwann cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - HOG