RNA isolation / purification Cells Cancer cell lines

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Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Products Thermo Fisher Scientific CM-H2DCFDA (General Oxidative Stress Indicator)

Get tips on using Carboxy-H2DCFDA (general oxidative stress indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Products Thermo Fisher Scientific Carboxy-H2DCFDA (general oxidative stress indicator)

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

RNA siRNA / miRNA gene silencing Human Jurkat GAPDH Lipid

RNA Microarray Human Precision cut lung slices Expression array

RNA siRNA / miRNA gene silencing Human HUVEC ATF4 Lipid

RNA siRNA / miRNA gene silencing Human HepG2 AMPKα1/α2

RNA siRNA / miRNA gene silencing Human CAL-27 ITGB5

RNA siRNA / miRNA gene silencing Human HT-1080 RELA

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