siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using QIAquick Gel Extraction Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using MinElute Reaction Cleanup Kit (250) to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using MinElute Gel Extraction Kit (250) to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - SiHa HPV-16

Get tips on using ChargeSwitch PCR Clean-Up Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski HPV-16

Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis HCT 116