siRNA / miRNA gene silencing Human PANC-1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1

Get tips on using Hs_KEAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - BEAS-2B KEAP1

Get tips on using MIF siRNA (h) to perform siRNA / miRNA gene silencing Human - Caki-2 MIF

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - rh-36 GLI1

Get tips on using Hs_HDAC2_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H HDAC2_1

Get tips on using Hs_HDAC1_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H HDAC1_6

Get tips on using Hs_BAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H BAP1_5

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H BAP1_3