siRNA / miRNA gene silencing Human HaCaT

Get tips on using SMARTpool: siGENOME PAK4 siRNA to perform siRNA / miRNA gene silencing Human - OVCAR-3 PAK4

Get tips on using siRNA Junction plakoglobin (SASI_Hs01_00246520) to perform siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Get tips on using Notch 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 Notch 1

Get tips on using NGFR p75 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC NGFR p75

Get tips on using AMPKα1/2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HepG2 AMPKα1/α2

Get tips on using CREB-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC ATF4 Lipid

Get tips on using SignalSilence® SAPK/JNK siRNA to perform siRNA / miRNA gene silencing Human - KGN SAPK/JNK

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.