Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human umbilical cord tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer
Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - PANC-1 human pancriatic cancer
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - PC-3 human prostate cancer
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - 3T3 L1 mouse adipose tissue
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - SK-MEL-2 human melanoma
Get tips on using Gentra Puregene Buccal Cell Kit (100) to perform DNA isolation / purification Cells - Primary cells Buccal cells
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
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