siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LNCap SKIL

Get tips on using siRNA PIN1 to perform siRNA / miRNA gene silencing Human - LNCap PIN1

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - DuCaP SKIL

Get tips on using siRNA PIN1 to perform siRNA / miRNA gene silencing Human - DU145 PIN1

Get tips on using siRNA MXD3 to perform siRNA / miRNA gene silencing Human - DAOY MXD3

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1

Get tips on using siRNA P300 to perform siRNA / miRNA gene silencing Human - BOSC23 p300

Get tips on using siRNA CBP to perform siRNA / miRNA gene silencing Human - BOSC23 CBP

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.