Immunohistochemistry Collagen Type VII Rabbit

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line DU145

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line PC3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit skeletal muscle cells

Get tips on using Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO) to perform Immunohistochemistry Human - E-Cadherin

Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10