siRNA / miRNA gene silencing Human Colon-26

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - IMR-90 CDC7

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - PANC-1 PKN3

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - PANC-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - PANC-1 hENT1

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - Capan-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - Capan-1 hENT1

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - SW1990 DNMT1/3b

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.