siRNA / miRNA gene silencing Human K562

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - Colon-26 PKN3

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - IMR-90 CDC7

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - PANC-1 PKN3

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - PANC-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - PANC-1 hENT1

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - Capan-1 CDC7

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - Capan-1 hENT1

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - SW1990 DNMT1/3b

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.