DNA Damage Assay Human bronchial epithelial cells (hBE)

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Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Products Promega Renilla luciferase vector, pGL4.74

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Products Cell Biolabs OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - Human Lung

Products Qiagen AllPrep DNA/RNA/miRNA Universal Kit

Get tips on using AllPrep DNA/RNA/Protein Mini Kit to perform Protein isolation Tissue - Human placental tissue

Products Qiagen AllPrep DNA/RNA/Protein Mini Kit

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products Sigma-Aldrich In Situ Cell Death Detection Kit, Fluorescein

Get tips on using Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit to perform Cell cycle assay human - FaDu

Products Thermo Fisher Scientific Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Tissue blood / plasma

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Tissue fecal sample

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Neuro2a

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines L02

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

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