DNA quantification

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HEK 293T

Get tips on using Promega PCR Master Mix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using PerfeCTa MultiPlex qPCR SuperMix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using QIAGEN Multiplex PCR Kit to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay MCF7

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)