Microarray Gene expression arrays Mouse liver tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using RNAprotect Tissue Reagent (250 ml) to perform RNA stabilization Sorted cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Rat_Renal tissue

Get tips on using Bac-to-Bac™ Baculovirus Expression System to perform Protein expression and purification Insect cells - Sf9 Drosha

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Get tips on using Allprotect Tissue Reagent (100 ml) to perform Stabilization of DNA Cervico-vaginal smears

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - immortalized Neuro2a

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse brain tissue