Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Get tips on using Comet SCGE assay kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)
Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - bone marrow mononuclear cells
Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells
Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hESC-EC
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse C166
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse 4T1
Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-2-EC
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment