siRNA / miRNA gene silencing Human OV2008 Yap Gene

Get tips on using Estrogen Receptor alpha siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC ERα

Get tips on using Tlr4 Mouse siRNA Oligo Duplex to perform siRNA / miRNA gene silencing Mouse - BV2 TLR4

Get tips on using ON-TARGETplus Mouse Lgals3 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 LGAL3S3

Get tips on using ON-TARGETplus Mouse Samhd1 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs SAMHD1

Get tips on using ON-TARGETplus Mouse Stat3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 STAT3

Get tips on using ON-TARGETplus Mouse Prkaa1 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Prkaa1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Silencer® Select_ MYD88 to perform siRNA / miRNA gene silencing Human - A375 MYD88

Get tips on using Silencer® Select_ TRIF to perform siRNA / miRNA gene silencing Human - A375 TRIF

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP