Western blot 1,4 β-N-acetyl-D-glucosamine

Get tips on using RPMI 1640, 1X to perform Mammalian cell culture media LNCaP

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media Ku812

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media U937

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media PNT1A

Get tips on using EBMTM Endothelial Cell Growth Basal Medium, 500 mL to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human peripheral blood mononuclear cells (PBMCs)

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media HL-60

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media CML T1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.