Live / Dead assay

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An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay yeast Urediniospore

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Syspastospora parasitica

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - MC3T3

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Saprolegnia diclina

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Phytophthora parasitica

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay bacteria - Staphylococcus aureus

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat cardiomyocytes

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat MSC

Products Abcam Live and Dead Cell Assay (Abcam)

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - mouse keratinocytes

Products Abcam Live and Dead Cell Assay (Abcam)

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells HUVEC
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