No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 19 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| • Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. |
Detection of Mycoplasma in cell cultures is performed as follows: • Viable mycoplasma in a test sample (cell culture supernatant) are lysed
• The released mycoplasmal enzymes react with the MycoAlert® Substrate
• The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® Reagent.
• Measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® Substrate (read B), a ratio can be determined that indicates the presence or absence of mycoplasma. |
|
| Upstream tips |
| • Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. |
| Protocol tips |
Detection of Mycoplasma in cell cultures is performed as follows: • Viable mycoplasma in a test sample (cell culture supernatant) are lysed
• The released mycoplasmal enzymes react with the MycoAlert® Substrate
• The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® Reagent.
• Measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® Substrate (read B), a ratio can be determined that indicates the presence or absence of mycoplasma. |
| Upstream tips |
Protocol tips |
Downstream tips |
• Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR.
•MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. These include the six species that account for 95% of all contaminations: M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, and A. laidlawii. |
• MycoStrip™ is able to detect as low as 10-102 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~107 CFU/ml. |
|
| Upstream tips |
• Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR.
•MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. These include the six species that account for 95% of all contaminations: M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, and A. laidlawii. |
| Protocol tips |
| • MycoStrip™ is able to detect as low as 10-102 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~107 CFU/ml. |
| Upstream tips |
Protocol tips |
Downstream tips |
| • The Resistance Plus MG FleXible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of M. genitalium and four 23S rRNA mutations associated with macrolide resistance. |
• Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions |
• Analyze data using GeneXpert Dx system version 5.1 software |
| Upstream tips |
| • The Resistance Plus MG FleXible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of M. genitalium and four 23S rRNA mutations associated with macrolide resistance. |
| Protocol tips |
| • Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions |
| Downstream tips |
| • Analyze data using GeneXpert Dx system version 5.1 software |
| Upstream tips |
Protocol tips |
Downstream tips |
• The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture.
• The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. |
• Prepare a 3% agarose gel.
• Prepare samples: Add 10 µL of the PCR product to 1.5 µL loading buffer |
|
| Upstream tips |
• The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture.
• The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. |
| Protocol tips |
• Prepare a 3% agarose gel.
• Prepare samples: Add 10 µL of the PCR product to 1.5 µL loading buffer |
| Upstream tips |
Protocol tips |
Downstream tips |
• The MycoSEQ Mycoplasma Detection Kit is a real-time PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7.
• The assay's sensitivity has been demonstrated by internal and external validations to detect 10 cfu/mL or the genomic equivalent of 10 GC/mL as recommended by regulatory guidance. |
• PCR Method: qPCR
• Detection Method: SYBR |
• Anlayze samples with AccuSEQ™ Software v3.1 on the QuantStudio™ 5 Instrument. |
| Upstream tips |
• The MycoSEQ Mycoplasma Detection Kit is a real-time PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7.
• The assay's sensitivity has been demonstrated by internal and external validations to detect 10 cfu/mL or the genomic equivalent of 10 GC/mL as recommended by regulatory guidance. |
| Protocol tips |
• PCR Method: qPCR
• Detection Method: SYBR |
| Downstream tips |
| • Anlayze samples with AccuSEQ™ Software v3.1 on the QuantStudio™ 5 Instrument. |
| Upstream tips |
Protocol tips |
Downstream tips |
• In the PrepSEQ 1-2-3 kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology.
|
• The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency.
• The protocol is recommended for use with a starting sample volume of 100 µL with a maximum cell density of 106 cells. |
|
| Upstream tips |
• In the PrepSEQ 1-2-3 kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology.
|
| Protocol tips |
• The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency.
• The protocol is recommended for use with a starting sample volume of 100 µL with a maximum cell density of 106 cells. |
| Upstream tips |
Protocol tips |
Downstream tips |
| • The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid, and simple fluorescence microscopic assay for the visual identification of mycoplasma infection in laboratory cell cultures. |
• In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope. |
|
| Upstream tips |
| • The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid, and simple fluorescence microscopic assay for the visual identification of mycoplasma infection in laboratory cell cultures. |
| Protocol tips |
| • In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope. |
| Upstream tips |
Protocol tips |
Downstream tips |
• The LookOut Mycoplasma PCR Detection Kit is highly sensitive, with a detection limit of only 2 genomes per μl.
•Taking advantage of the highly specific JumpStart Taq DNA Polymerase and a proprietary primer design, false positives are greatly reduced. |
• Technique: PCR
• The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. |
|
| Upstream tips |
• The LookOut Mycoplasma PCR Detection Kit is highly sensitive, with a detection limit of only 2 genomes per μl.
•Taking advantage of the highly specific JumpStart Taq DNA Polymerase and a proprietary primer design, false positives are greatly reduced. |
| Protocol tips |
• Technique: PCR
• The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. |
| Upstream tips |
Protocol tips |
Downstream tips |
| • Highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. |
• The EZ-PCR™ Mycoplasma Detection Kit includes an internal amplification control, a plasmid containing a non-mycoplasma-specific DNA sequence to test for false negatives. |
|
| Upstream tips |
| • Highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. |
| Protocol tips |
| • The EZ-PCR™ Mycoplasma Detection Kit includes an internal amplification control, a plasmid containing a non-mycoplasma-specific DNA sequence to test for false negatives. |
| Upstream tips |
Protocol tips |
Downstream tips |
• PlasmoTest™ features two major components:
• The mycoplasma sensor cells, HEK-Blue™-2 cells
- the HEK-Blue™ Detection medium |
• Sensitivity: detects 5.102-5.105 cfu/ml depending on the species of mycoplasma
• No false positive: a positive result indicates the presence of a cell culture contaminant |
|
| Upstream tips |
• PlasmoTest™ features two major components:
• The mycoplasma sensor cells, HEK-Blue™-2 cells
- the HEK-Blue™ Detection medium |
| Protocol tips |
• Sensitivity: detects 5.102-5.105 cfu/ml depending on the species of mycoplasma
• No false positive: a positive result indicates the presence of a cell culture contaminant |
| Upstream tips |
Protocol tips |
Downstream tips |
| • They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. |
• The positive control for the MycoAlert® Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set. |
|
| Upstream tips |
| • They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. |
| Protocol tips |
| • The positive control for the MycoAlert® Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set. |
| Upstream tips |
Protocol tips |
Downstream tips |
• abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours.
• Kit is able to detect as low as 10 copies of Mycoplasma /sample |
• Positive control is included in the kit |
|
| Upstream tips |
• abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours.
• Kit is able to detect as low as 10 copies of Mycoplasma /sample |
| Protocol tips |
| • Positive control is included in the kit |
| Upstream tips |
Protocol tips |
Downstream tips |
| • The MycoTOOL Mycoplasma Real-Time PCR Kit is designed to test at least 10 cell culture samples for the absence of Mycoplasma. |
• Automated workflow using automated sample preparation and Real-Time PCR
• <5 hours, including sample preparation (data on file)
|
|
| Upstream tips |
| • The MycoTOOL Mycoplasma Real-Time PCR Kit is designed to test at least 10 cell culture samples for the absence of Mycoplasma. |
| Protocol tips |
• Automated workflow using automated sample preparation and Real-Time PCR
• <5 hours, including sample preparation (data on file)
|
| Upstream tips |
Protocol tips |
Downstream tips |
| • cobas® TV/MG for use on the cobas® 6800/8800 Systems is an automated, qualitative in vitro diagnostic test, that utilizes real-time polymerase chain reaction (PCR), for the direct detection of Trichomonas vaginalis (TV) and/or Mycoplasma genitalium (MG) DNA |
|
|
| Upstream tips |
| • cobas® TV/MG for use on the cobas® 6800/8800 Systems is an automated, qualitative in vitro diagnostic test, that utilizes real-time polymerase chain reaction (PCR), for the direct detection of Trichomonas vaginalis (TV) and/or Mycoplasma genitalium (MG) DNA |
| Upstream tips |
Protocol tips |
Downstream tips |
• MYCOPLASMA IST 3 combines enumeration, identification and antibiotic susceptibility testing for detection of Mycoplasma hominis and Ureaplasma spp.
• Complete diagnosis in one step
• Simple, secure and convenient |
|
|
| Upstream tips |
• MYCOPLASMA IST 3 combines enumeration, identification and antibiotic susceptibility testing for detection of Mycoplasma hominis and Ureaplasma spp.
• Complete diagnosis in one step
• Simple, secure and convenient |
| Upstream tips |
Protocol tips |
Downstream tips |
| • CE-IVD-marked multiplex real-time PCR assay that detects M.genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance |
|
• Analyze data using Seegene viewer software version 1.6 |
| Upstream tips |
| • CE-IVD-marked multiplex real-time PCR assay that detects M.genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance |
| Downstream tips |
| • Analyze data using Seegene viewer software version 1.6 |
| Upstream tips |
Protocol tips |
Downstream tips |
| • Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlert™ Kit allowing the use of less sensitive plate luminometers or multifunctional readers. |
|
|
| Upstream tips |
| • Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlert™ Kit allowing the use of less sensitive plate luminometers or multifunctional readers. |
| Upstream tips |
Protocol tips |
Downstream tips |
| • Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. |
• Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase |
|
| Upstream tips |
| • Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. |
| Protocol tips |
| • Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase |
| Upstream tips |
Protocol tips |
Downstream tips |
| • M. genitalium - 23S rRNA gene; 5 mutations: A2058G, A2059G, A2058T, A2058C, A2059C, associated with macrolide-resistance by melt-curve analysis |
|
|
| Upstream tips |
| • M. genitalium - 23S rRNA gene; 5 mutations: A2058G, A2059G, A2058T, A2058C, A2059C, associated with macrolide-resistance by melt-curve analysis |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!