Mammalian cell culture media HEM

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Human Melanocyte Media

Cell Applications Inc

Upstream tips	Protocol tips	Downstream tips
	Human epidermal melanocytes (HEM) were obtained from Cell Applications, Inc. (San Diego, CA, USA) (Cat. No. 104K-05a), maintained in melanocyte growth medium (Cell Applications, Inc.), incubated at 37°C in a 5% CO2 humidified incubator, and expanded for at least five passages. The medium was changed twice a week and subcultured when it was about 80% confluent.

Protocol tips
Human epidermal melanocytes (HEM) were obtained from Cell Applications, Inc. (San Diego, CA, USA) (Cat. No. 104K-05a), maintained in melanocyte growth medium (Cell Applications, Inc.), incubated at 37°C in a 5% CO2 humidified incubator, and expanded for at least five passages. The medium was changed twice a week and subcultured when it was about 80% confluent.

Manufacturer protocol Publication protocol 1 Relevant paper

Medium 254CF, Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.

Protocol tips
supplement M-254 medium with HMGS-2, penicillin (100 U/ml), gentamicin (10 µg/ml) and amphotericin B (0.25 µg/ml) and culture the cells at 37°C in 5% CO2.

Manufacturer protocol Publication protocol 1 Relevant paper

MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM

Lonza

Upstream tips	Protocol tips	Downstream tips
	melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD)

Protocol tips
melanocyte growth media (MGM) supplemented with melanocyte growth factors (MGF) (Lonza Walkersville Inc, Walkersville, MD)

Publication protocol 1 Relevant paper

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