No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 4 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).
|
Protocol tips |
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
Downstream tips |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).
|
Upstream tips |
Protocol tips |
Downstream tips |
- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT.
- To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number. |
To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation. |
|
Upstream tips |
- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT.
- To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number. |
Protocol tips |
To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation. |
Upstream tips |
Protocol tips |
Downstream tips |
|
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step
|
|
Protocol tips |
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step
|
Upstream tips |
Protocol tips |
Downstream tips |
|
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
|
Protocol tips |
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!