DNA isolation / purification Cells

Get tips on using DNeasy UltraClean 96 Microbial Kit (384) to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using DNeasy PowerSoil HTP 96 Kit (384) to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using DNeasy PowerSoil HTP 96 Kit (384) to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using NucleoSpin® Soil to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using QIAamp MinElute Media Kit to perform DNA isolation / purification Tissue - genital / cervical samples

Get tips on using QIAamp UCP Pathogen Mini Kit to perform DNA isolation / purification Tissue - bronchoalveolar lavage

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Tissue - placenta

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.