rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Cells - immortalized HCA2

Get tips on using QIAamp Viral RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Vero

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Vagina

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Mouse Trachea