Site Directed Mutagenesis (SDM) Human Insertion IMR-32

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human SKBR3

Get tips on using GeneChip™ Human Genome U133A 2.0 Array to perform Microarray Comperative genomic hybridization - Human Tumor

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCAEC

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCtASMC

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCtAEC

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - A549

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using EasySep™ Direct Human B Cell Isolation Kit to perform Cell Isolation B cell

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.