siRNA / miRNA gene silencing Human Aortic smooth muscle cell

Get tips on using SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit to perform Microarray Human - PCOS

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Lamb muscle tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse skeletal muscle

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Mouse skeletal muscle cells

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mouse aortic endothelial cells (MAECs)

Get tips on using Tissue or Cell Total Protein Extraction Kit to perform Protein isolation Tissue - Lamb muscle tissue

Get tips on using pMIR-REPORT™ miRNA Expression Reporter Vector System to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells