siRNA / miRNA gene silencing Human SKOV-3

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HESC Lipofectamine

Get tips on using connexin 43 ShRNA to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray RNA amplification & Labeling - Mouse brain tissue Biotin

Get tips on using JAK1 Antibody (B-3): sc-376996 to perform Western blotting Jak1

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

Get tips on using TFIIB Antibody (D-3): sc-271736 to perform ChIP Anti-bodies TFIIB

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid

Get tips on using IncuCyte® Caspase-3/7 Apoptosis Assay Reagent to perform Apoptosis assay cell type - Caspase 3/7