siRNA / miRNA gene silencing Human Primary Human Hepatocytes

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GeneChip® Human Genome U133 Plus 2.0 Array Product

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Human endometrial stromal cells Biotin

Products Thermo Fisher Scientific GeneChip® Human Genome U133 Plus 2.0 Array

mirVana™ miRNA Isolation Kit, with phenol Product

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody Product

Get tips on using Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Estrogen receptor (ER) - Rabbit Human -NA-

Products Ventana Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody

RNA isolation / purification Cells - primary porcine primary chondrocytes Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine primary chondrocytes

Lipofectamine® RNAiMAX Transfection Reagent Product

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - OV-2008 Lipofectamine