siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Get tips on using HIF-1α siRNA (m) to perform siRNA / miRNA gene silencing Mouse - AtT20 Hif-1alpha

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human osteoblasts

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a c-Jun

Get tips on using HuR siRNA to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR Lipid

Get tips on using In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

Get tips on using Rn_Pdcd8_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 AIF/Pdcd8

Get tips on using Net siRNA (m) to perform siRNA / miRNA gene silencing Mouse - FL83B Elk3/Net

Get tips on using ATG5 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Atg5