siRNA / RNAi /miRNA transfection Human Cells Primary splenocytes

Get tips on using MITF siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 MITF

Get tips on using TLR4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR4

Get tips on using C23 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a C23

Get tips on using ANT2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ANT2

Get tips on using iBONi siRNA pool to perform siRNA / miRNA gene silencing Mouse - B16-F10 P53

Get tips on using Nrf2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Get tips on using Atf4 siRNA(m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using STEAP2 metalloreductase to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) STEAP2