DNA isolation / purification Cells Immortalized cell lines

Get tips on using AllPrep DNA/RNA FFPE Kit to perform RNA isolation / purification Tissue - Mouse Prostate

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Stomach

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Retina

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using MagNA Pure Compact Nucleic Acid Isolation Kit I to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human preadipocytes

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.