ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - Human Gut

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Esophagus

Get tips on using PAXgene Tissue RNA Kit to perform RNA isolation / purification Tissue - Human Colon

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - OVCAR-5

Get tips on using FITC BrdU Flow Kit to perform Cell cycle assay human - THP-1

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - MCF 10A

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - bone marrow mononuclear cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.