siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells

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ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type human umbelical vein endothelial cells (HUVEC)

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells

Products Qiagen miRNeasy Mini kit

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary porcine primary chondrocytes

Products Thermo Fisher Scientific PureLink™ RNA Mini Kit

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling HCT116, HTC15 human colon cancer cells

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Products Santa Cruz Biotechnology Glut1 siRNA and shRNA Plasmids (h)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Products Santa Cruz Biotechnology CD74 siRNA and shRNA Plasmids (h)

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Mouse Brain

Products Macherey Nagel NucleoSpin® miRNA

Cell culture media 3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta

Products Macherey Nagel NucleoSpin® miRNA

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