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Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19 Experiment

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Human fetal osteoblastic (hFOB) 1.19

CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm Product

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - SK-MEL-1

Products Cell Biolabs CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm

3D Cell Culture Media Human primary breast ephitelial cells-organoids Experiment

Cell culture media 3D Cell Culture Media Human primary breast ephitelial cells-organoids

3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres Experiment

Cell culture media 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids Experiment

Cell culture media 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

3D Cell Culture Media Mouse primary lung epithelial cells-organoids Experiment

Cell culture media 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres Experiment

Cell culture media 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

3D Cell Culture Media Primary human breast tumors-Mammospheres Experiment

Cell culture media 3D Cell Culture Media Primary human breast tumors-Mammospheres

3D Cell Culture Media Human blood-brain barrier organoid Experiment

Cell culture media 3D Cell Culture Media Human blood-brain barrier organoid

Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit Product

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis HeLa

Products Thermo Fisher Scientific Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

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