rna-isolation-purification-cells-immortalized-mda-mb-361

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using E.Z.N.A.® Yeast RNA Kit to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using E.Z.N.A.® Fungal RNA Kit to perform RNA isolation / purification Yeast - Aspergillus nidulans

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Spleen