Site Directed Mutagenesis (SDM) Hamster Point mutation BHK-21

- Found 4505 results

Reporter gene assay β-galactosidase substrates - BHK-21 baby hamster kidney cells Experiment

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates BHK-21 baby hamster kidney cells

Site Directed Mutagenesis (SDM) Human - Deletion MDA-MB-231 sodium channel β1 subunit Experiment

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Deletion MDA-MB-231 sodium channel β1 subunit

Phusion Site-Directed Mutagenesis Kit Product

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Rat - Deletion H9C2 SRF