RNA isolation / purification Cells Cancer cell lines

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using E.Z.N.A.® Yeast RNA Kit to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using E.Z.N.A.® Fungal RNA Kit to perform RNA isolation / purification Yeast - Aspergillus nidulans

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Spleen

Get tips on using AllPrep DNA/RNA FFPE Kit to perform RNA isolation / purification Tissue - Mouse Prostate