Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line SW480
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Get tips on using DNA-free™ DNA Removal Kit to perform
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - Mouse liver tissue
Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - Mouse cardiac tissue
Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Tissue - ME epithelial tissue
Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary porcine tracheal epithelial cells
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