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Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Intestine

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Human Gut

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Get tips on using PureLink ™ miRNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using MEDOX-Easy Total RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Mouth

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines