Stabilization of DNA

Get tips on using QIAGEN Multiplex PCR Kit to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay MCF7

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MCF10A

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - BJ

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - BMDM

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - BMDM

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HeLa

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Brain tissue

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.