Live / Dead assay yeast

Get tips on using LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry to perform Live / Dead assay yeast - Candida albicans

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Syspastospora parasitica

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Saprolegnia diclina

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Phytophthora parasitica

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Clostridium sporogenes

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Candida albicans

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay bacteria - Staphylococcus aureus

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.