Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RIP3

Get tips on using siRNA Junction plakoglobin (SASI_Hs01_00246520) to perform siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Human CRISP-3 Antibody to perform Immunohistochemistry Human - CRISP3