Protein Expression Eukaryotic cells T. thermophila

Get tips on using RGS·His HRP Conjugate Kit to perform Protein tag Detection of His-tagged proteins

Get tips on using TAGZyme Kit to perform Protein tag His-tag removal

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Chlamydia pneumoniae

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus anthracis

Get tips on using Qproteome Mitochondria Isolation Kit to perform Protein enrichment Mitochondria

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.